Zymo-Seq RiboFree Total RNA Library Kit

The kit instructions are very well written so that its easy to follow the procedure. Moreover I had a doubt about the rRNA depletion incubation time (because some of my samples had low RNA concentrations), I wrote to the support and their reply was fast and useful.

We had a great experience in using this specific sequencing library kit. We tried the free trial kit with great success. Despite some technical issues with the protocol due to some inaccurate descriptions in the manual, Zymo was very kind and highly supportive to replace the wasted kits. The tech support was always very responsive. We have successfully generated RNA-Seq libraries for a good number of RNA samples and have done sequencing with Illumina NovaSeq (150bp PE) or NextSeq (75bp PE) platforms with great sequencing yields. The ribosomal RNA removal part is very easy to do with either human or mouse RNA using the same protocol. Both sequencing platforms have generated consistent and excellent sequencing reads with high quality. The sequencing work was done with our internal core or external commercial vendors. The unique dual index worked very well and the samples were covered equally. We highly recommend this special and easy kit for Total RNA-Seq.

The kit was very easy to use as the protocol is very well written and easy to follow. I used it for extracting bacterial RNA from honey bee guts and it worked very well, especially for the samples in which the bacteria concentration was higher.