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His-Spin Protein Miniprep
Zymo Research
SKU / CAT#: P2001
$7400
usd
$7.40/Preps
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P2001

His-Spin Protein Miniprep

Zymo Research Corporation
4reviews
4
20citationscitations
20
19questionsquestions
19
SKU / CAT#: P2001
MPN: P2003-2
$7400
usd
$7.40/Preps
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Available
SHIPS May 15th
Available
SHIPS May 15th
SKU
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Description
The His-Spin Protein Miniprep provides a fast spin-column based purification technology for His-tagged proteins. Up to 1 mg of His-tagged protein can be purified in 5 minutes and eluted in as little as 100 µl of His-Elution Buffer. The purified protein is ultra-pure and can be used directly for enzymatic assays, protein biochemical analyses, SDS-PAGE and other sensitive applications. The straightforward spin, wash, and elute protocol dramatically simplifies protein purification and allows the end user to get results in minutes, not hours.
The His-Spin Protein Miniprep provides a fast spin-column based purification technology for His-tagged proteins. Up to 1 mg of His-tagged protein can be purified in 5 minutes and eluted in as little as 100 µl of His-Elution Buffer. The purified protein is ultra-pure and can be used directly for enzymatic assays, protein biochemical analyses, SDS-PAGE and other sensitive applications. The straightforward spin, wash, and elute protocol dramatically simplifies protein purification and allows the end user to get results in minutes, not hours.
Shipping & Handling
Shipped In
Ambient
Safety & Storage
Storage Temperature
See product data sheet, protocol and/or package labeling for storage conditions.
Safety Statement
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
Regulatory & Compliance
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Technical Documents
MSDS (2)
His-Spin Protein Miniprep - SDS (MSDS)
His-Spin Protein Miniprep - SDS (MSDS)
PROTOCOL (1)
His-Spin Protein Miniprep - Protocol
Specifications
CLASS
Protein
SUBCLASS
Protein Purification
SHIPPING NOTES
All orders received before 3:00 pm Pacific Standard Time Monday through Friday will be shipped the same day via FedEx® Ground delivery unless otherwise stated or requested.
COUNTRY OF ORIGIN
United States of America
*USAGE / SAFETY STATEMENT
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
PURITY
Electrophoretically pure. Purified high-quality protein is suitable for enzyme kinetics, protein biochemical analyses, SDS-PAGE, and other applications.
MATERIAL
Nickel charged agarose
KIT COMPONENTS
● His-Affinity Gel (P2003-2). _x000D_● His-Binding Buffer (P2003-3). _x000D_● His-Wash Buffer (P2003-4). _x000D_● His-Elution Buffer (P2003-5). _x000D_● Collection Tubes (C1001-50). _x000D_● Zymo-Spin P1 Columns (P2003-1). _x000D_
ELUTION VOLUME
Imidazole gradient
EQUIPMENT
Microcentrifuge
Questions & Answers (0)
Reviews & Ratings (4)
5.0 out of 5 stars
5
100%
4
0%
3
0%
2
0%
1
0%
The product works as described. Easy to follow protocol (easier than most!). Storage is simple and seems to not affect efficacy. I DO recommend.
Ian
InitiateBio, LLC
This kit is awesome!
Leyla
Cemvita Factory
The product enables simple and fast His-Tag purification for small volumes. For the purification of proteins produced within our cell-free expression system the...
Marc
Saarland University
Product is very easy to use, purification is done quickly, protein was very pure and active afterwards.
Viktoria
Albert-Ludwigs-Universität Freiburg
The product works as described. Easy to follow protocol (easier than most!). Storage is simple and seems to not affect efficacy. I DO recommend.
Ian
InitiateBio, LLC
This kit is awesome!
Leyla
Cemvita Factory
The product enables simple and fast His-Tag purification for small volumes. For the purification of proteins produced within our cell-free expression system the...
Marc
Saarland University
Product is very easy to use, purification is done quickly, protein was very pure and active afterwards.
Viktoria
Albert-Ludwigs-Universität Freiburg
Nov. 16, 2021
Crucial for simplistic HIS tag purification!!
Ian C.
InitiateBio, LLC
The product works as described. Easy to follow protocol (easier than most!). Storage is simple and seems to not affect efficacy. I DO recommend.
Mar. 19, 2021
Great results.
Leyla F.
Cemvita Factory
This kit is awesome!
Dec. 11, 2019
Simple and fast protein purification!
Marc F.
Saarland University
The product enables simple and fast His-Tag purification for small volumes. For the purification of proteins produced within our cell-free expression system the kit is a valuable tool.
Citations (0)
His-Spin Protein Miniprep
His-Spin Protein Miniprep
Shipping & Handling
Shipped In
Ambient
Safety & Storage
Storage Temperature
See product data sheet, protocol and/or package labeling for storage conditions.
Safety Statement
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
Regulatory & Compliance
Badge
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