ZymoBIOMICS Microbial Community Standard II (Log Distribution)

Very informative mock. We used it several times, with different PCR primers, and managed to get reproducible results. We can evaluate the propensity of PCR to over/under amplify bacteria, and its ability to recover a heavily unbalanced community without shifting the proportions too much. We observed a slight difference towards the expected relative abundance, but this is exactly what needs to be checked before analyzing your own data. We are working with Staphylococcus aureus, and even if its only present in 0.0001% we still managed to get the signal, meaning if we do have S.aureus in our samples, the PCR will be sensitive enough.

It was so useful to evaluate my amplicon smaples. The usage was simple so I was able to use it very easily.

I achieve high quality reproducible results. The composition appears to be consistent with each lot I have bought, which is very helpful in the preparation and quality control of making our libraries.