Specifications
SUBCLASS
High-Throughput/Automated Isolation
SHIPPING NOTES
All orders received before 3:00 pm Pacific Standard Time Monday through Friday will be shipped the same day via FedEx® Ground delivery unless otherwise stated or requested.
COUNTRY OF ORIGIN
United States of America
APPLICATION
All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
*USAGE / SAFETY STATEMENT
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
PURITY
High quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing
SOURCE
Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water.
KIT COMPONENTS
● ZymoBIOMICS DNA Wash Buffer 2 (D4300-4-200). _x000D_● ZymoBIOMICS DNase/RNase Free Water (D4302-5-10). _x000D_● ZymoBIOMICS MagWash 2 (D4302-4-200). _x000D_● ZymoBIOMICS MagWash 1 (D4302-3-100). _x000D_● ZymoBIOMICS HRC Prep Solution (D4300-7-30). _x000D_● ZymoBIOMICS DNA Binding Buffer (D4300-2-250). _x000D_● ZymoBIOMICS DNA Wash Buffer 1 (D4300-3-100). _x000D_● ZymoBIOMICS Lysis Solution (D4300-1-150). _x000D_● ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) (S6012-50). _x000D_● Silicon-A-HRC Plate (C2009). _x000D_● ZR-96 BashingBead Lysis Rack (S6002-96-3). _x000D_● 96-Well Plate Cover Foil (C2007-4). _x000D_● Zymo-Spin I-96 Plate (C2004). _x000D_● 96-Well Block (P1001-2). _x000D_
ELUTION VOLUME
≥ 20 µl DNA Elution Buffer or DNase free water
EQUIPMENT
Centrifuge w/ microplate carriers, 96-well plate/block disruptor or pulverizer
SIZE RANGE
Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield.
YIELD
Up to 5 µg total DNA can be eluted into 20 µl