Quick-cfDNA Serum & Plasma Kit provides a simple and reliable method for the rapid preparation of high-quality circulating cell-free DNA from serum, plasma, amniotic fluid, cerebrospinal fluid (CSF), and saliva. A combination of chemical and enzymatic methods are used to efficiently recover total DNA (including cell-free apoptotic, necrotic, mitochondrial and viral DNA) linearly from up to 10 ml of sample (Reference Figure A). Zymo-Spin technology allows for ultra-pure DNA to be eluted in as little as 35 μl water. The resulting DNA is suitable for all subsequent analyses and molecular manipulations...
Quick-cfDNA Serum & Plasma Kit provides a simple and reliable method for the rapid preparation of high-quality circulating cell-free DNA from serum, plasma, amniotic fluid, cerebrospinal fluid (CSF), and saliva. A combination of chemical and enzymatic methods are used to efficiently recover total DNA (including cell-free apoptotic, necrotic, mitochondrial and viral DNA) linearly from up to 10 ml of sample (Reference Figure A). Zymo-Spin technology allows for ultra-pure DNA to be eluted in as little as 35 μl water. The resulting DNA is suitable for all subsequent analyses and molecular manipulations...
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Safety & Storage
Storage Temperature
See product data sheet, protocol and/or package labeling for storage conditions.
Safety Statement
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
All orders received before 3:00 pm Pacific Standard Time Monday through Friday will be shipped the same day via FedEx® Ground delivery unless otherwise stated or requested.
COUNTRY OF ORIGIN
United States of America
*USAGE / SAFETY STATEMENT
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
PURITY
High-quality DNA is ready for all sensitive downstream applications such as qPCR and Next-Generation sequencing.
SOURCE
Serum, plasma, amniotic fluid, and cerebrospinal fluid (CSF).
KIT COMPONENTS
● DNA Elution Buffer (D3004-4-4). _x000D_● S&P 5X Digestion Buffer (D4076-1-32). _x000D_● S&P DNA Binding Buffer (D4076-2-170). _x000D_● S&P DNA Prep Buffer (D4076-3-10). _x000D_● S&P DNA Wash Buffer (Concentrate) (D4076-4-12). _x000D_● Collection Tubes (C1001-50). _x000D_● Zymo-Spin III-S Columns w/ LuerLock Caps and 15 ml Reservoir (C1049-25). _x000D_● Proteinase K w/ Storage Buffer Set (D3001-2-125). _x000D_
EQUIPMENT
Water bath or heat block (55ºC), microcentrifuge, vacuum/vacuum manifold or swinging bucket centrifuge.
Questions & Answers (0)
Reviews & Ratings (4)
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The cfRNA extraction kit from Zymo is very well designed! We even compared it with more traditional methods like the Trizol protocol. This kit from Zymo outperformed...
Huong
International University, Vietnam.
I have tested several similar kits, but this kit provides the highest cfDNA yield with a wide range of fragments.
Alex
University
Good value for money, high cfDNA yields for a column based method.
Paula
RMH
I have tried several circulating nucleic acid isolation kits and this solution is my favorite so far, with the highest yield and with the widest length range of...
Alex
University
The cfRNA extraction kit from Zymo is very well designed! We even compared it with more traditional methods like the Trizol protocol. This kit from Zymo outperformed...
Huong
International University, Vietnam.
I have tested several similar kits, but this kit provides the highest cfDNA yield with a wide range of fragments.
Alex
University
Good value for money, high cfDNA yields for a column based method.
Paula
RMH
I have tried several circulating nucleic acid isolation kits and this solution is my favorite so far, with the highest yield and with the widest length range of...
Alex
University
Awesome result!
Huong H.
International University, Vietnam.
The cfRNA extraction kit from Zymo is very well designed! We even compared it with more traditional methods like the Trizol protocol. This kit from Zymo outperformed the Trizol method in all aspects, from ease of use to final RNA product quality. In fact, we published a paper on this result.
Please check out this link:
https://link.springer.com/chapter/10.1007/978-3-030-75506-5_50
Great cfDNA yield.
Alex K.
University
I have tested several similar kits, but this kit provides the highest cfDNA yield with a wide range of fragments.
Great kit, clean manual cfDNA prep.
Paula P.
RMH
Good value for money, high cfDNA yields for a column based method.
Citations (0)
Shipping & Handling
Shipped In
Ambient
Safety & Storage
Storage Temperature
See product data sheet, protocol and/or package labeling for storage conditions.
Safety Statement
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
Regulatory & Compliance
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.