The ZR BAC DNA Miniprep Kit efficiently isolates BAC plasmid DNA or other large plasmids (e.g., PAC) from E. Coli using a procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol with color-coded reagents that allow easy visualization of complete bacterial cell lysis and neutralization. The innovative Zymo-Spin™ IC-XL columns are optimized for high yield, endotoxin-free plasmid DNA recovery. BAC DNA purified using the ZR BAC DNA Miniprep Kit is ideal for sequencing, PCR, endonuclease digestion, etc.
The ZR BAC DNA Miniprep Kit efficiently isolates BAC plasmid DNA or other large plasmids (e.g., PAC) from E. Coli using a procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol with color-coded reagents that allow easy visualization of complete bacterial cell lysis and neutralization. The innovative Zymo-Spin™ IC-XL columns are optimized for high yield, endotoxin-free plasmid DNA recovery. BAC DNA purified using the ZR BAC DNA Miniprep Kit is ideal for sequencing, PCR, endonuclease digestion, etc.
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See product data sheet, protocol and/or package labeling for storage conditions.
Safety Statement
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
All orders received before 3:00 pm Pacific Standard Time Monday through Friday will be shipped the same day via FedEx® Ground delivery unless otherwise stated or requested.
APPLICATION
Transfection, transformation, sequencing, restriction endonuclease digestion, PCR, in vitro transcription, and other sensitive applications.
*USAGE / SAFETY STATEMENT
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
Up to 10 µg per preparation, depending on the plasmid copy number, initial volume of E. Coli culture processed, and culture growth conditions. The typical yield is 1 µg per 3 ml of culture.
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I use this BAC miniprep kit all the time with gram negative bacteria containing plasmids of a variety of sizes (from 3kb up to 200kb). I can easily see all of the...
Erin
University of Idaho
I use this BAC miniprep kit all the time with gram negative bacteria containing plasmids of a variety of sizes (from 3kb up to 200kb). I can easily see all of the...
Erin
University of Idaho
Very useful kit for collecting all bacterial plasmids and chromosome.
Erin M.
University of Idaho
I use this BAC miniprep kit all the time with gram negative bacteria containing plasmids of a variety of sizes (from 3kb up to 200kb). I can easily see all of the plasmids as well as the chromosome when running a few microliters of the eluted samples on an agarose gel. I get good results with PCR on these samples as well. There is definitely an issue with residual ethanol after sample elution but a 10-minute incubation at 55C solves this problem. You could also try an extra spin after the last wash (using an empty collection tube) if you dont want to heat your samples.
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Shipping & Handling
Shipped In
Ambient
Safety & Storage
Storage Temperature
See product data sheet, protocol and/or package labeling for storage conditions.
Safety Statement
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
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