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Zymoprep Yeast Plasmid Miniprep II
Zymo Research
SKU / CAT#: D2004
$13800
usd
$2.76/Preps
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D2004

Zymoprep Yeast Plasmid Miniprep II

Zymo Research Corporation
257reviews
257
20citationscitations
20
9questionsquestions
9
SKU / CAT#: D2004
MPN: E1004
$13800
usd
$2.76/Preps
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Available
SHIPS May 12th
Available
SHIPS May 12th
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Description
The Zymoprep Yeast Plasmid Miniprep II is a simple and efficient yeast plasmid miniprep that is based on the E. coli alkaline lysis method but using Zymolyase as the first solution. The kit provides all the necessary reagents for plasmid isolation from S. cerevisiae, C. albicans and S. pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The procedure is simple and efficient, with no need for glass beads or phenol. The Zymoprep II recovers about 5 fold more plasmid DNA compared to Zymoprep I and eliminates the isopropanol precipitation step. Reliably recover plasmid...
The Zymoprep Yeast Plasmid Miniprep II is a simple and efficient yeast plasmid miniprep that is based on the E. coli alkaline lysis method but using Zymolyase as the first solution. The kit provides all the necessary reagents for plasmid isolation from S. cerevisiae, C. albicans and S. pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The procedure is simple and efficient, with no need for glass beads or phenol. The Zymoprep II recovers about 5 fold more plasmid DNA compared to Zymoprep I and eliminates the isopropanol precipitation step. Reliably recover plasmid...
Shipping & Handling
Shipped In
Ambient
Safety & Storage
Storage Temperature
See product data sheet, protocol and/or package labeling for storage conditions.
Safety Statement
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
Regulatory & Compliance
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Technical Documents
MSDS (1)
Zymoprep Yeast Plasmid Miniprep II - SDS (MSDS)
PROTOCOL (1)
Zymoprep Yeast Plasmid Miniprep II - Protocol
Specifications
CLASS
DNA
SUBCLASS
DNA Purification
SHIPPING NOTES
All orders received before 3:00 pm Pacific Standard Time Monday through Friday will be shipped the same day via FedEx® Ground delivery unless otherwise stated or requested.
COUNTRY OF ORIGIN
United States of America
APPLICATION
Plasmid DNA is well suited for downstream applications such as PCR, transformation, hybridization and other sensitive applications
*USAGE / SAFETY STATEMENT
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
SOURCE
Cell Culture (Colonies/Patches or Liquid Culture)
KIT COMPONENTS
● Zymolyase (E1004). _x000D_● Solution 1 Digestion Buffer (D2004-1-10). _x000D_● Solution 2 Lysis Buffer (D2004-2-10). _x000D_● Solution 3 Neutralizing Buffer (D2004-3-20). _x000D_● DNA Wash Buffer (Concentrate) (D4003-2-6). _x000D_● Collection Tubes (C1001-50). _x000D_● Zymo-Spin I Columns (C1003-50). _x000D_
ELUTION VOLUME
≥ 10 µl
EQUIPMENT
Microcentrifuge & Heat Block/Bath
SIZE RANGE
Up to 23 kb.
YIELD
Typically between 0.01-0.3 ng for most 2 µ based plasmids from 1.5 ml overnight cultures
Questions & Answers (0)
Reviews & Ratings (6)
4.8 out of 5 stars
5
50%
4
50%
3
0%
2
0%
1
0%
Its an easy of use product, good balance between value and money
Rene
univerisity
Easy to use, with great results.
Sofia
UNAM
DirectZol is super easy to use and I routinely get excellent quality total RNA (e.g., RINs 9-10).
Matthew
Northern Arizona University
I love Zymo products and I cannot wait to open my own lab someday and use exclusively Zymo reagents. I have never had issues with their products and this RNA miniprep...
Vincent
UConn Health
I used the Direct Zol RNA miniprep kit to isolate total RNA from my bacterial cultures. I was able to receive a high yield with no problem and the kit only takes...
Jyoti
Miami University
This kit produces high quality RNA, which can be concentrated using a small volume of elution buffer; no need concentrating afterwards.
Vincent
Delaware State University
Its an easy of use product, good balance between value and money
Rene
univerisity
Easy to use, with great results.
Sofia
UNAM
DirectZol is super easy to use and I routinely get excellent quality total RNA (e.g., RINs 9-10).
Matthew
Northern Arizona University
I love Zymo products and I cannot wait to open my own lab someday and use exclusively Zymo reagents. I have never had issues with their products and this RNA miniprep...
Vincent
UConn Health
I used the Direct Zol RNA miniprep kit to isolate total RNA from my bacterial cultures. I was able to receive a high yield with no problem and the kit only takes...
Jyoti
Miami University
This kit produces high quality RNA, which can be concentrated using a small volume of elution buffer; no need concentrating afterwards.
Vincent
Delaware State University
Mar. 31, 2022
It is good
Rene N.
univerisity
Its an easy of use product, good balance between value and money
Mar. 30, 2022
Good results in short time
Sofia P.
UNAM
Easy to use, with great results.
Mar. 29, 2022
By far the easiest method Ive used.
Matthew S.
Northern Arizona University
DirectZol is super easy to use and I routinely get excellent quality total RNA (e.g., RINs 9-10).
Citations (0)
Zymoprep Yeast Plasmid Miniprep II
Zymoprep Yeast Plasmid Miniprep II
Shipping & Handling
Shipped In
Ambient
Safety & Storage
Storage Temperature
See product data sheet, protocol and/or package labeling for storage conditions.
Safety Statement
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
Regulatory & Compliance
Badge
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