Acetylcholine transferase synthesizes the neurotransmitter acetylcholine (ACh) from choline and acetyl CoA. ACh is packaged and stored into synaptic vesicles until its release. ACh is hydrolyzed by acetylcholine esterase (AChE) to yield choline and acetate. Choline is taken up by the high affinity VAChT/VAT located at the membrane and recycled for synthesis of ACh.
Applications:
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.
Recommended Dilution:
Western Blot: 1-10ug/ml using ECL.
ELISA: 0.5-1ug/ml. Control...
Acetylcholine transferase synthesizes the neurotransmitter acetylcholine (ACh) from choline and acetyl CoA. ACh is packaged and stored into synaptic vesicles until its release. ACh is hydrolyzed by acetylcholine esterase (AChE) to yield choline and acetate. Choline is taken up by the high affinity VAChT/VAT located at the membrane and recycled for synthesis of ACh.
Applications:
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.
Recommended Dilution:
Western Blot: 1-10ug/ml using ECL.
ELISA: 0.5-1ug/ml. Control...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
-20°C
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
Specifications
CLASS
Polyclonal Antibodies
SUBCLASS
Transport Proteins
APPLICATION
E
*USAGE / SAFETY STATEMENT
For laboratory research and development purposes only.
PURITY
Purified by immunoaffinity chromatography.
PHYSICAL FORM
Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.
GRADE
Affinity Purified
ANTIBODY TYPE
Pab
HOST SPECIES
mouse
ISOTYPE
IgY
CROSS REACTIVITY
Mo Rt
IMMUNOGEN
A 20aa peptide mapping within the C-terminus, cytoplasmic domain of rat/mouse VAT conjugated to KLH. Species sequence homology: human-93%, monkey-91%.
SPECIFIC ACTIVITY
Recognizes mouse Vesicular Acetylcholine Transporter. Species Crossreactivity: rat. No significant sequence homology is seen with other neurotransmitter transporter.
COMMODITY CODE
30021010
HOST
chicken
Questions & Answers (0)
Citations (0)
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
-20°C
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.