Applications:
Suitable for use in Western Blot, Immunocytochemistry and Kinase assays. Other applications not tested.
Recommended Dilution:
Western Blot: Detected Mps1 in HeLa total cell extract.
Immunocytochemistry: Has been reported by an independent laboratory to show positive immunostaining for Mps1 in U2OS or HeLa cells permeabilized with 0.5% Triton-X-100 and fixed with methanol or paraformaldehyde.
Kinase Assay: Has been reported by an independent laboratory to immunoprecipitate Mps1 from HeLa total cell extract. The immunoprecipitates were then used in a radioactive kinase assay.
Optimal...
Applications:
Suitable for use in Western Blot, Immunocytochemistry and Kinase assays. Other applications not tested.
Recommended Dilution:
Western Blot: Detected Mps1 in HeLa total cell extract.
Immunocytochemistry: Has been reported by an independent laboratory to show positive immunostaining for Mps1 in U2OS or HeLa cells permeabilized with 0.5% Triton-X-100 and fixed with methanol or paraformaldehyde.
Kinase Assay: Has been reported by an independent laboratory to immunoprecipitate Mps1 from HeLa total cell extract. The immunoprecipitates were then used in a radioactive kinase assay.
Optimal...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
4°C Do Not Freeze
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
Specifications
CLASS
Monoclonal Antibodies
SUBCLASS
Protein Kinases
APPLICATION
IC WB
CLONE NUMBER
5H8 (3-472-1)
ACCESSION#
NM_003318
*USAGE / SAFETY STATEMENT
For laboratory research and development purposes only.
PURITY
Purified by Protein G affinity chromatography.
PHYSICAL FORM
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
GRADE
Affinity Purified
ANTIBODY TYPE
Mab
HOST SPECIES
human
ISOTYPE
IgG1
CROSS REACTIVITY
Hu
IMMUNOGEN
His-tagged fusion protein corresponding to aa3-856 of human Mps1.
SPECIFIC ACTIVITY
Recognizes human Mps1, Mr ~97kD. Does not react with mouse.
SWISS PROT#
P33981
COMMODITY CODE
30021010
HOST
mouse
Questions & Answers (0)
Citations (0)
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
4°C Do Not Freeze
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
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