Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: detected LKB1 in RIPA lysates from Rat2 cells. Rat2 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with L3185-03 (1ug/ml). Proteins were visualized using a IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Immunocytochemistry: detected LKB1 in transfected Cos-7 cells fixed with 3-7% paraformaldehyde and permeabilized with 0.1% Triton X-100.
Optimal dilutions to be determined by the researcher.
*Recommended...
Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: detected LKB1 in RIPA lysates from Rat2 cells. Rat2 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with L3185-03 (1ug/ml). Proteins were visualized using a IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Immunocytochemistry: detected LKB1 in transfected Cos-7 cells fixed with 3-7% paraformaldehyde and permeabilized with 0.1% Triton X-100.
Optimal dilutions to be determined by the researcher.
*Recommended...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
4°C Do Not Freeze
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
Specifications
CLASS
Monoclonal Antibodies
SUBCLASS
Protein Kinases
APPLICATION
IC WB
CLONE NUMBER
5H64
ACCESSION#
NM_000455
*USAGE / SAFETY STATEMENT
For laboratory research and development purposes only.
PURITY
Purified by Protein G affinity chromatography.
PHYSICAL FORM
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
GRADE
Affinity Purified
ANTIBODY TYPE
Mab
HOST SPECIES
human
ISOTYPE
IgG
CROSS REACTIVITY
Hu Mo Rt
IMMUNOGEN
GST fusion protein corresponding to residues 1-433 of human LKB1.
SPECIFIC ACTIVITY
Recognizes human LKB1 at ~51kD. An additional protein was also detected at ~90kD. Species Crossreactivity: mouse and rat
SWISS PROT#
Q15831
COMMODITY CODE
30021010
HOST
mouse
Questions & Answers (0)
Citations (0)
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
4°C Do Not Freeze
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
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