Applications:
Suitable for use in Western Blot, Immunocytochemistry and Peptide inhibition. Other applications not tested.
Recommended Dilution:
Western Blot: 0.05-0.4ug/ml detects phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells treated with colcemid, but not unmodified recombinant Histone H3. Acid extract from untreated and colcemid treated HeLa cells, along with recombinant Histone H3 were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-Histone H3 (Ser10) (0.1ug/ml). Proteins were visualized using a goat anti-mouse secondary...
Applications:
Suitable for use in Western Blot, Immunocytochemistry and Peptide inhibition. Other applications not tested.
Recommended Dilution:
Western Blot: 0.05-0.4ug/ml detects phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells treated with colcemid, but not unmodified recombinant Histone H3. Acid extract from untreated and colcemid treated HeLa cells, along with recombinant Histone H3 were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-Histone H3 (Ser10) (0.1ug/ml). Proteins were visualized using a goat anti-mouse secondary...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
4°C Do Not Freeze
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
Specifications
CLASS
Monoclonal Antibodies
SUBCLASS
Histones
APPLICATION
IC WB
CLONE NUMBER
3H10
*USAGE / SAFETY STATEMENT
For laboratory research and development purposes only.
PURITY
Purified by Protein G affinity chromatography.
PHYSICAL FORM
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
GRADE
Affinity Purified
ANTIBODY TYPE
Mab
HOST SPECIES
human
ISOTYPE
IgG1,k
CROSS REACTIVITY
Hu
IMMUNOGEN
Proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3
SPECIFIC ACTIVITY
Recognizes histone H3 phosphorylated at Ser10, Mr 17kD. Species Crossreactivity: Human. Broad species crossreactivity is expected.
SWISS PROT#
Q16695
COMMODITY CODE
30021010
HOST
mouse
Questions & Answers (0)
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Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
4°C Do Not Freeze
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
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