Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Western Blot: 0.5-4ug/ml detects HDAC3 in nuclear extracts from HeLa cells.
Immunoprecipitation: 4ug immunoprecipitates HDAC3 from 500ug of HeLa nuclear extract, followed by immunoblot analysis using H5109-48F.
Immunoprecipitation/HDAC: 3-15ug immunoprecipitates HDAC3 from 500ug of a HeLa cell nuclear extract. Enzymatic activity was assessed using H5109-62.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Store product at 4°C in the dark....
Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Western Blot: 0.5-4ug/ml detects HDAC3 in nuclear extracts from HeLa cells.
Immunoprecipitation: 4ug immunoprecipitates HDAC3 from 500ug of HeLa nuclear extract, followed by immunoblot analysis using H5109-48F.
Immunoprecipitation/HDAC: 3-15ug immunoprecipitates HDAC3 from 500ug of a HeLa cell nuclear extract. Enzymatic activity was assessed using H5109-62.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Store product at 4°C in the dark....
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
4°C Do Not Freeze
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
Specifications
CLASS
Monoclonal Antibodies
SUBCLASS
Enzymes, Deacetylase
APPLICATION
IP WB
CLONE NUMBER
4H330 (3G6)
ACCESSION#
NM_003883
*USAGE / SAFETY STATEMENT
For laboratory research and development purposes only.
PURITY
Purified by Protein G affinity chromatography.
PHYSICAL FORM
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
GRADE
Affinity Purified
ANTIBODY TYPE
Mab
HOST SPECIES
human
ISOTYPE
IgG2a
CROSS REACTIVITY
Bo Hm Hu Mo
IMMUNOGEN
Synthetic peptide (NEFYDGDHDNDKESDVEI) corresponding to amino acids 411-428 of human HDAC3. Conjugated to KLH. The immunizing sequence is identical in mouse, rat and chicken.
SPECIFIC ACTIVITY
Recognizes human HDAC3, MW 49kD. Species Crossreactivity: mouse, bovine and hamster. Species Sequence Homology: rat and chicken.
SWISS PROT#
O15379
COMMODITY CODE
30021010
HOST
mouse
Questions & Answers (0)
Citations (0)
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
4°C Do Not Freeze
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
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