SPECIFIC ACTIVITY
The pore region of HCN1 (HCNP15-P) is very conserved among various HCNs. HCN1: mouse, rat, human, rabbit=100%; HCN2: mouse, rat, human (86%); HCN3: mouse (95%), rat (90%); HCN4: rat, human (95%). The HCNP15-P sequence is also conserved in insect (Helothis virescens, 78%), Drosophila (78%) and Sea urchin (S. purpuratus, 69%) HCNs. Therefore, antibodies to HCNP15-P should react with most HCNs and related sequences. Antibody reactivity in various species is not known. The HCNP15-P control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity. This antibody may be useful in studying various HCNs and to examine the possibility of immunoblocking the pore region and impact on the functionality of HCNs proteins.
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