Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in aa metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:23,000
Western Blot: 0.03-0.1ug/ml, observed in human kidney and liver lysates on ~45kD bands
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May...
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in aa metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:23,000
Western Blot: 0.03-0.1ug/ml, observed in human kidney and liver lysates on ~45kD bands
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
-20°C
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
Specifications
SUBCLASS
Fatty Acid Binding Protein
APPLICATION
E WB
ACCESSION#
NP_002071.2
*USAGE / SAFETY STATEMENT
For laboratory research and development purposes only.
PURITY
Purified by immunoaffinity chromatography.
PHYSICAL FORM
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
GRADE
Affinity Purified
ANTIBODY TYPE
Pab
HOST SPECIES
human
ISOTYPE
IgG
CROSS REACTIVITY
Hu Mo Rt
IMMUNOGEN
Synthetic peptide corresponding to CKDADEAKRVES, from human GOT2, at the internal region of the protein (NP_002071.2).
SPECIFIC ACTIVITY
Recognizes human GOT2. Species sequence homology: Mouse and rat.
SWISS PROT#
P00505
COMMODITY CODE
30021010
HOST
goat
CLASS
Polyclonal Antibodies
Questions & Answers (0)
Citations (0)
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
-20°C
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.