It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced “p29” and “24K” protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 0.5-1ug/million...
It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced “p29” and “24K” protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 0.5-1ug/million...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
-20°C
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
Specifications
CLASS
Monoclonal Antibodies
SUBCLASS
Heat Shock Proteins
APPLICATION
E
CLONE NUMBER
G3.1
*USAGE / SAFETY STATEMENT
For laboratory research and development purposes only.
PURITY
Purified by Protein A/G affinity chromatography.
PHYSICAL FORM
Supplied as a liquid in 10mM PBS. Also available with BSA and azide, see 168796.
GRADE
Affinity Purified
ANTIBODY TYPE
Mab
HOST SPECIES
human
ISOTYPE
IgG1,k
CROSS REACTIVITY
Hu
IMMUNOGEN
Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells. Cellular Localization: Predominantly cytoplasmic with some nuclear
SPECIFIC ACTIVITY
Recognizes human HSP27. Species Crossreactivity: chimpanzee, sheep, rat, mouse, and chicken.
COMMODITY CODE
30021010
HOST
mouse
Questions & Answers (0)
Citations (0)
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
-20°C
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.