ATG5 Antibody: ATTO 594

Polyclonal

Blue Ice or 4ºC. All orders received in North America by 12:00 PM PST Monday to Thursday will usually be shipped out on the same day. North American customers will typically receive their orders within 1-2 business days. All shipments are F.O.B. from point of shipment, pre-paid and added to the invoice. Freight charges include but not limited to: priority service and packaging materials.Products are shipped with “cold packs” in insulated containers. There is a charge for this packaging and for the shipping costs via FedEx. Please inspect packages immediately upon receipt and inform Labscoop Customer Support of any shortage or damage within 48 hours of receipt. We can only accept returns after we have given prior authorization and issued specific shipping instructions. If a customer wishes to use their own FedEx account number please indicate this on the purchase order or during checkout.

Canada

WB, ICC/IF

https://www.ncbi.nlm.nih.gov/gene/9474

NP_001273035.1

Non-hazardous

Non-hazardous

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

1 mg/ml

WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Rabbit

Human

ATTO 594

Peptide Affinity Purified

ISO 9001:2015

ATG5

Synthetic peptide from the mid-protein of human ATG5

Predicted molecular weight at ~32.4kDa. Observed molecular weights at ~48-60kDa based on ATG12-ATG5 heterodimer.

PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated

https://antibodyregistry.org/search.php?q=AB_2704911

Cytoplasm

ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (1). Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. ATG5 is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II (2).

1. Pyo J.O., et al. (2005) J Biol Chem. 280(21): 20722-20729. 2. Mizushima N., et al. (2003) Mol Biol Cell. 15(3): 1101-1111.

Cancer | Apoptosis | Cancer | Autophagy | Cardiovascular System | Heart | Cell Signaling | Metabolism | Metabolism processes

APG5-like Antibody, ASP Antibody, hAPG5 Antibody