Specifications
SHIPPING NOTES
Blue Ice or 4ºC. All orders received in North America by 12:00 PM PST Monday to Thursday will usually be shipped out on the same day. North American customers will typically receive their orders within 1-2 business days. All shipments are F.O.B. from point of shipment, pre-paid and added to the invoice. Freight charges include but not limited to: priority service and packaging materials.Products are shipped with “cold packs” in insulated containers. There is a charge for this packaging and for the shipping costs via FedEx. Please inspect packages immediately upon receipt and inform Labscoop Customer Support of any shortage or damage within 48 hours of receipt. We can only accept returns after we have given prior authorization and issued specific shipping instructions. If a customer wishes to use their own FedEx account number please indicate this on the purchase order or during checkout.
APPLICATION
WB, IHC, ICC/IF
GENE ID
https://www.ncbi.nlm.nih.gov/gene/2670
*USAGE / SAFETY STATEMENT
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
ANTIBODY TYPE
WB (1:1000); optimal dilutions for assays should be determined by the user.
REACTIVITY SPECIES
Human | Mouse | Rat
PURIFICATION
Protein G Purified
QUALITY CERTIFICATION / COMPLIANCE
ISO 9001:2015
IMMUNOGEN
Synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP; 100% identical in rat and mouse
SPECIFICITY
Detects ~50kDa. Cross-reacts with GFAP-R416W and other GFAP mutant proteins.
STORAGE BUFFER
PBS pH 7.4, 50% glycerol, 0.1% sodium azide *Storage buffer may change when conjugated
ANTIBODY ID
https://antibodyregistry.org/search.php?q=AB_2701686
CELLUAR LOCALIZATIONS
Cytoplasm
SCIENTIFIC BACKGROUND
The 50 kDa type III intermediate filament protein glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes. GFAP associates with the calcium binding protein annexin II-p2 and S-100. Association with these proteins together with phosphorylation regulates GFAP polymerization. Astroycytes respond to brain injury by proliferatin (astrogliosis), and one of the first events to occur during astrocyte proiliferation is increased GFAP expression. Interestingly, antibodies to GFAP have been detected in individuals with dementia.
REFERENCES
1. Velasco M.E., et al. (1980) Cancer. 45:484.
2. Bonnin J.M., et al. (1984) Acta Neuropathology. 62:185.
3. Lee VM-Y., et al. (1984) J. Neurochem. 42:25-32 (1984).
4. Trojanowski JQ et al. (1986) J. Neurochem. 6(3): 650-660 (1986).
5. Schmidt ML et al; Lab Invest 56:282-294 (1987).
6. Kosik KS et al; Neuron 1:817-825 (1988).
7. Schmidt ML et al; Lab Invest 59:460-466 (1988).
8. Mokuna, K, et al: J Neurosci Res 23:396 (1989).
9. Molenaar, et al; Exp Neurology 108:1-9 (1990).
10. Tohyama T et al; Am J Pathol, 142:871-882 (1993).
11. Tohyama T et al; Am J Pathol 142:883-892 (1993).
12. Thilenius, A.R.B., et al; J. Immunol. 162(2): 643-650 (1999).
RESEARCH AREA
Neuroscience | Cell Markers | Glial Markers | Astrocyte Markers
ALTERNATIVE NAMES
Glial fibrillary acidic protein Antibody, Intermediate filament protein Antibody, Astrocyte Antibody, gfapl Antibody, DKFZp459C0729 Antibody, MGC139638 Antibody, FLJ45472 Antibody, AI836096 Antibody, GFAP Antibody