Description
A modified version of Ham’s F-12 medium, Ham’s F-12K (Kaighn’s) Medium was originally formulated for use with or without supplemental serum. Adding components like putrescine, thymidine, hypoxanthine, zinc, and higher levels of sodium pyruvate and amino acids than found in traditional media, can compensate for the lack of serum. Depending on the cell type cultured however, FBS and growth factors are often added to the medium.
HMF-BBZ-XXX-000 contains 292 mg/L L-glutamine and 2500 mg/L sodium bicarbonate.
A modified version of Ham’s F-12 medium, Ham’s F-12K (Kaighn’s) Medium was originally formulated for use with or without supplemental serum. Adding components like putrescine, thymidine, hypoxanthine, zinc, and higher levels of sodium pyruvate and amino acids than found in traditional media, can compensate for the lack of serum. Depending on the cell type cultured however, FBS and growth factors are often added to the medium.
HMF-BBZ-XXX-000 contains 292 mg/L L-glutamine and 2500 mg/L sodium bicarbonate.
Specifications
SUBCLASS
Classical & Basal Cell Culture Media
SHIPPING NOTES
Inspect your packages upon receipt and notify us promptly of any damage or discrepancies. Should an error occur, we will work with you to find an appropriate remedy to the problem. Upon obtaining prior authorization, we will fulfill requests to exchange products that do not meet expectations or specifications, although due to the nature of our products this is only possible for standard stock items. All requests for a return must be made within 60 days from the time the product is received. Any shipping charges incurred in returning products mis-shipped by us will be covered by us, while all other return shipping fees are the responsibility of the customer.
COUNTRY OF ORIGIN
United States
*USAGE / SAFETY STATEMENT
This product is produced for in-vitro use and is suitable for research and manufacturing only. It is the end user’s responsibility to determine the suitability and obtain sufficient validation for each application of use.
REGULATORY NOTICE & RESTRICTIONS
Produced in an FDA Registered facility.
STERILITY
Sterilized by 0.2 um filtration system
QUALITY CERTIFICATION / COMPLIANCE
Manufactured at our GMP compliant and FDA registered facility. Manufacturer underwent the International Serum Industry Association (ISIA)’s rigorous independent audit of our processes and earned the organization’s Certificate of Traceability.
APPEARANCE
Red Transparent Solution
FORMULATION
CaCl2 (anhydrous) - 135.24 mg/L
KH2PO4 - 58.523 mg/L
CuSO4·5H2O - 0.0025 mg/L
FeSO4·7H2O - 0.834 mg/L
KCl - 283.29 mg/L
MgCl2·6H2O - 105.716 mg/L
MgSO4 - 192.64 mg/L
NaCl - 7597.2 mg/L
NaHCO3 - 2500 mg/L
Na2HPO4 (anhydrous) - 115.02 mg/L
ZnSO4·7H2O - 0.14375 mg/L
D-Glucose - 1260 mg/L
HEPES - 0 mg/L
Hypoxanthine·Na - 4.083 mg/L
Linoleic Acid - 0 mg/L
DL-6,8-Thioctic Acid - 0.02063 mg/L
Phenol Red - 3.31806 mg/L
Putrescine·HCl - 0.3222 mg/L
Sodium Pyruvate - 220 mg/L
Thymidine - 0.7266 mg/L
L-Alanine - 17.818 mg/L
L-Arginine·HCl - 421.4 mg/L
L-Asparagine·H2O - 30.02 mg/L
L-Aspartic Acid - 26.62 mg/L
L-Cysteine·HCl·H2O - 70.24 mg/L
L-Glutamic Acid - 29.42 mg/L
L-Glutamine - 292.2 mg/L
Glycine - 15.014 mg/L
L-Histidine·3HCl·H2O - 41.92 mg/L
L-Isoleucine - 7.872 mg/L
L-Leucine - 26.24 mg/L
L-Lysine·HCl - 73.04 mg/L
L-Methionine - 8.952 mg/L
L-Phenylalanine - 9.912 mg/L
L-Proline - 69.06 mg/L
L-Serine - 21.02 mg/L
L-Threonine - 23.82 mg/L
L-Tryptophan - 4.084 mg/L
L-Tyrosine·2Na·2H2O - 10.872 mg/L
L-Valine - 23.42 mg/L
Biotin - 0.07329 mg/L
Calcium D-pantothenate - 0.477 mg/L
Choline Chloride - 13.96 mg/L
Folic Acid - 1.3242 mg/L
Myo-Inositol - 18.02 mg/L
Niacinamide - 0.03663 mg/L
Pyridoxine HCI - 0.06168 mg/L
Riboflavin - 0.03764 mg/L
Thiamine HCI - 0.3373 mg/L
Vitamin B12 - 13.96 mg/L
OSMOLALITY
320 - 355 mOsm/kg H2O