NCED3 / Anti-9-Cis-Epoxycarotenoid Dioxygenase NCED3, Chloroplastic Antibody

Primary Antibodies

Crop Antibodies

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

United States

Research Use Only.

Lyophilized

66 kDa

Rabbit Polyclonal Antibody

Serum

Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.

KLH-conjugated synthetic peptide (14 aa from C terminal section) derived from Oryza sativa NCED3 (Os03g0645900).(Os03g0645900, Q5MBR5)

Oryza sativa, Panicum virgatum, Sorghum bicolor, Setaria viridis, Zea mays, Triticum aestivum, Hordeum vulgare

Reconstitution with 150µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

66 kDa

Recom: 2.5 ng, 10 ng and 25 ng recombinant protein containing the peptide for immunization and having a molecular mass of 52 kDa. Electrophoresis: 12% SDS-PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1 h. Blocking: 5% skim milk at RT or 4℃ for 1 h. Primary antibody: 1:1000 dilution overnight at 4℃. Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000). Detection: using chemiluminescence substrate and image were captured with CCD camera.