HSP17.6C / Anti-17.6 KDa Class I Heat Shock Protein 3 Antibody

Primary Antibodies

Arabidopsis Antibodies

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

United States

Research Use Only.

Lyophilized

18 kDa

Rabbit Polyclonal Antibody

Serum

Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.

KLH-conjugated synthetic peptide (16 aa from Central section) derived from Arabidopsis thaliana HSP17.6C (AT1G53540).(AT1G53540, P13853)

Arabidopsis thaliana, Triticum aestivum, Hordeum vulgare, Glycine max, Populus, trichocarpa, Vitis vinifera, Brassica napus, Solanum tuberosum, Solanum lycopersicum, Brassica rapa, Spinacia oleracea, Gossypium raimondii, Panicum virgatum, Setaria viridis

Reconstitution with 150 µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

18 kDa

Recom: 2.5 ng, 10 ng and 25 ng recombinant protein containing the peptide for immunization and having a molecular mass of 45 kDa. Electrophoresis: 12% SDS-PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1 h. Blocking: 5% skim milk at RT or 4℃ for 1 h. Primary antibody: 1:1000 dilution overnight at 4℃. Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000). Detection: using chemiluminescence substrate and image were captured with CCD camera.