CO / Anti-Zinc Finger Protein CONSTANS Antibody

Primary Antibodies

Arabidopsis Antibodies

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

United States

Research Use Only.

Lyophilized

42 kDa

Mouse Monoclonal Antibody

Immunogen Affinity Purified

Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.

Recombinant protein (110-300 aa) derived from Arabidopsis thaliana CO (AT5G15840).(AT5G15840, Q39057)

Arabidopsis thaliana

Reconstitution with 150 µl of 0.01 M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

42 kDa

Example 1 Nuc-WT: 6 μg nuclear protein from WT of Arabidopsis thaliana. Nuc-co: 6 μg nuclear protein from co mutant of Arabidopsis thaliana. Electrophoresis: 12% SDS-PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1 h. Blocking: 5% skim milk at RT or 4℃ for 1 h. Primary antibody: 1:1000 dilution overnight at 4℃. Secondary antibody: 1:5000 dilution using Goat Anti-Mouse IgG H&L(HRP) (Cat# PHY6006). Detection: using chemiluminescence substrate and image were captured with CCD camera. Example 2 Recom: 1 ng and 10 ng recombinant protein containing the peptide for immunization and having a molecular mass of 32 kDa. Electrophoresis: 15% SDS-PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1 h. Blocking: 5% skim milk at RT or 4℃ for 1 h. Primary antibody: 1:1000 dilution overnight at 4℃. Secondary antibody: 1:5000 dilution using Goat Anti-Mouse IgG H&L(HRP) (Cat# PHY6006). Detection: using chemiluminescence substrate and image were captured with CCD camera.