Different classes of nonsymbiotic plant hemoglobins have been identified and divided into class 1 (Hb1) (AT2G16060) and class 2 (Hb2) based on phylogenetic characteristics, gene expression patterns, and oxygen-binding properties. Hb2 might act as an oxygen sensor or play a role in electron transfer, possibly to a bound oxygen molecule.
Different classes of nonsymbiotic plant hemoglobins have been identified and divided into class 1 (Hb1) (AT2G16060) and class 2 (Hb2) based on phylogenetic characteristics, gene expression patterns, and oxygen-binding properties. Hb2 might act as an oxygen sensor or play a role in electron transfer, possibly to a bound oxygen molecule.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
COUNTRY OF ORIGIN
United States
*USAGE / SAFETY STATEMENT
Research Use Only.
PHYSICAL FORM
Lyophilized
FREE CHARACTERISTICS
18 kDa
CLONALITY
Rabbit Polyclonal Antibody
PURIFICATION
Immunogen Affinity Purified
DILUTION
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
IMMUNOGEN
KLH-conjugated synthetic peptide (15 aa from C terminal section) derived from Arabidopsis thaliana Ahb2 (AT3G10520).(AT3G10520, O24521)
CONFIRMED SPECIFICITY & CROSS REACTIVITY
Arabidopsis thaliana
RECONSTITUTION
Reconstitution with 150 µl of 0.01 M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
EXPECTED / APPARENT MW
18 kDa
APPLICATION EXAMPLE
TP: 20 μg total protein from Arabidopsis thaliana. Electrophoresis: 15% SDS-PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1 h. Blocking: 5% skim milk at RT or 4℃ for 1 h. Primary antibody: 1:2000 dilution overnight at 4℃. Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L(HRP) (Cat# PHY6000). Detection: using chemiluminescence substrate and image were captured with CCD camera.
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Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Safety Statement
Research Use Only.
Regulatory & Compliance
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