WRKY18 is a pathogen-induced transcription factor. It binds W-box sequences in vitro. It forms protein complexes and with WRKY40 and WRKY60. WRKY18 (AT4G31800), WRKY40 (AT1G80840) and WRKY60 (AT2G25000) have partially redundant roles in response to the hemibiotrophic bacterial pathogen Pseudomonas syringae and the necrotrophic fungal pathogen Botrytis cinerea, with WRKY18 playing a more important role than the other two.
WRKY18 is a pathogen-induced transcription factor. It binds W-box sequences in vitro. It forms protein complexes and with WRKY40 and WRKY60. WRKY18 (AT4G31800), WRKY40 (AT1G80840) and WRKY60 (AT2G25000) have partially redundant roles in response to the hemibiotrophic bacterial pathogen Pseudomonas syringae and the necrotrophic fungal pathogen Botrytis cinerea, with WRKY18 playing a more important role than the other two.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Safety Statement
Research Use Only.
Regulatory & Compliance
Specifications
CLASS
Primary Antibodies
SHIPPING NOTES
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
COUNTRY OF ORIGIN
United States
*USAGE / SAFETY STATEMENT
Research Use Only.
PHYSICAL FORM
Lyophilized
SUBCLASS
Arabidopsis Antibodies
CLONALITY
Rabbit Polyclonal Antibody
PURIFICATION
Immunogen Affinity Purified
IMMUNOGEN
KLH-conjugated synthetic peptide (15 aa from N terminal section) derived from Arabidopsis thaliana WRKY18 (AT4G31800).(AT4G31800, Q9C5T4)
DILUTION
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
RECONSTITUTION
Reconstitution with 150 µl of 0.01M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
FREE CHARACTERISTICS
35 kDa
CONFIRMED SPECIFICITY & CROSS REACTIVITY
Arabidopsis thaliana
EXPECTED / APPARENT MW
35 kDa
APPLICATION EXAMPLE
Nuc: 3 μg and 6 μg nuclear protein from Arabidopsis thaliana, respectively. Electrophoresis: 15% SDS-PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1 h. Blocking: 5% skim milk at RT or 4℃ for 1 h. Primary antibody: 1:1000 dilution overnight at 4℃. Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L(HRP) (Cat# PHY6000). Detection: using chemiluminescence substrate and image were captured with CCD camera.
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Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Safety Statement
Research Use Only.
Regulatory & Compliance
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