Cu-Zn superoxide dismutase-1 (SOD-1) is a well-characterized cytosolic scavenger of oxygen free radicals that requires copper and zinc binding to potentiate its enzymatic activity. Enzymatically, SOD-1 facilitates the dismutation of oxygen radicals to hydrogen peroxide and also catalyzes pro-oxidant reactions, which include the peroxidase activity and hydroxyl radical generating activity. SOD-1 is ubiquitously expressed in somatic cells and functions as a homodimer. Defects in the gene encoding SOD-1 have been implicated in the progression of neurological diseases, including amyotrophic lateral...

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Safety & Storage

Storage Temperature

Aliquot the SOD1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Safety Statement

This SOD1 antibody is available for research use only.

Regulatory & Compliance

Technical Documents

Datasheet (1)

zAgaGsod1-antibody-sod1-4329-v9162

Specifications

CLASS

Primary Antibodies

SUBCLASS

Monoclonal

UNIPROT ID

P00441

CLONE NUMBER

SOD1/4329

*USAGE / SAFETY STATEMENT

This SOD1 antibody is available for research use only.

FORMAT

Purified

APPLICATION.1

FACS, WB, IHC-P

HOST SPECIES

Mouse

ISOTYPE

Mouse IgG2b, kappa

REACTIVITY SPECIES

Human

CLONALITY

Monoclonal (mouse origin)

PURIFICATION

Protein A/G affinity

IMMUNOGEN

A portion of amino acids 14-148 was used as the immunogen for the SOD1 antibody.

STORAGE BUFFER

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

APPLICATION DETAILS

Flow cytometry: 1-2ug/million cells,Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml

APPLICATION NOTES

Optimal dilution of the SOD1 antibody should be determined by the researcher.

LOCALIZATION

Cytoplasmic

Questions & Answers (0)

Citations (0)

Western blot testing of human 1) JEG-3 and 2 )LNCaP cell lysate using SOD1 antibody (clone SOD1/4329). Predicted molecular weight ~16 kDa.

FACS staining of PFA-fixed human MCF-7 cells with using SOD1 antibody (blue, clone SOD1/4331) and isotype control (red).

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SOD1 antibody (clone SOD1/4329). These results demonstrate the foremost specificity of the SOD1/4329 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Shipping & Handling

Shipped In

Cold Packs

Safety & Storage

Storage Temperature

Aliquot the SOD1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Safety Statement

This SOD1 antibody is available for research use only.

Regulatory & Compliance

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