It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Shipping & Handling

Shipped In

Cold Packs

Safety & Storage

Storage Temperature

Store the anti-Bromodeoxyuridine antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Safety Statement

This anti-Bromodeoxyuridine antibody is available for research use only.

Regulatory & Compliance

Technical Documents

Datasheet (1)

AFGeoanti-bromodeoxyuridine-antibody-spm166-v9115

Specifications

CLASS

Primary Antibodies

SUBCLASS

Monoclonal

UNIPROT ID

Not Applicable

CLONE NUMBER

SPM166

*USAGE / SAFETY STATEMENT

This anti-Bromodeoxyuridine antibody is available for research use only.

FORMAT

Purified

APPLICATION.1

IHC-P

HOST SPECIES

Mouse

ISOTYPE

Mouse IgG1, kappa

REACTIVITY SPECIES

All species

CLONALITY

Monoclonal (mouse origin)

PURIFICATION

Protein G affinity chromatography

IMMUNOGEN

Bromodeoxyuridine was used as the immunogen for this anti-Bromodeoxyuridine antibody.

STORAGE BUFFER

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

APPLICATION DETAILS

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

APPLICATION NOTES

Optimal dilution of the antibody should be determined by the researcher.

LOCALIZATION

Nuclear