Biotin / Vitamin B7 / Vitamin H Antibody
SKU / CAT#: V8868-20UG
$21900
usd
$10.95/UG
V8868-20UG

Biotin / Vitamin B7 / Vitamin H Antibody

NSJ Bioreagents
SKU / CAT#: V8868-20UG
MPN: V8868-20UG
$21900
usd
$10.95/UG
Available
SHIPS Jul 7th
Available
SHIPS Jul 7th
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Description
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Aliquot the Biotin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Safety Statement
This Biotin antibody is available for research use only.
Regulatory & Compliance
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Technical Documents
Specifications
CLASS
Primary Antibodies
SUBCLASS
Monoclonal
UNIPROT ID
Not Applicable
CLONE NUMBER
BTN399
*USAGE / SAFETY STATEMENT
This Biotin antibody is available for research use only.
FORMAT
Purified
APPLICATION.1
FACS, IF, WB, IHC-P
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG1, kappa
REACTIVITY SPECIES
All species
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
Protein A/G affinity
IMMUNOGEN
Biotinylated sheep immunoglobulin was used as the immuongen for the Biotin antibody.
STORAGE BUFFER
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
APPLICATION DETAILS
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
APPLICATION NOTES
Optimal dilution of the Biotin antibody should be determined by the researcher.
Questions & Answers (0)
Citations (0)
IHC staining of endogenous biotin in FFPE human kidney tissue with Biotin antibody (clone BTN399). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of endogenous biotin in FFPE human kidney tissue with Biotin antibody (clone BTN399). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Biotin antibody (clone BTN399) as confirmation of integrity and purity.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Aliquot the Biotin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Safety Statement
This Biotin antibody is available for research use only.
Regulatory & Compliance
Badge
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