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Prohibitin Antibody / PHB
SKU / CAT#: V7615SAF-100UG
$49900
usd
$4.99/UG
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V7615SAF-100UG

Prohibitin Antibody / PHB

NSJ Bioreagents
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SKU / CAT#: V7615SAF-100UG
MPN: V7615SAF-100UG
$49900
usd
$4.99/UG
Available
SHIPS May 12th
Available
SHIPS May 12th
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Description
This antibody recognizes a protein of 30kDa which is identified as Prohibitin (PHB), an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although PHB mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. It is an ideal mitochondrial marker. It shows anti-proliferative activity and...
This antibody recognizes a protein of 30kDa which is identified as Prohibitin (PHB), an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although PHB mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. It is an ideal mitochondrial marker. It shows anti-proliferative activity and...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the Prohibitin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This Prohibitin antibody is available for research use only.
Regulatory & Compliance
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Technical Documents
Datasheet (1)
YklSuprohibitin-antibody-phb-3225-protein-microarray-validated-v7615
Specifications
CLASS
Primary Antibodies
SUBCLASS
Monoclonal
UNIPROT ID
P35232
CLONE NUMBER
PHB/3225
*USAGE / SAFETY STATEMENT
This Prohibitin antibody is available for research use only.
FORMAT
Purified (Sodium Azide Free)
APPLICATION.1
WB, IF, IHC-P
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG2b, kappa
REACTIVITY SPECIES
Human
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
Protein G affinity chromatography
IMMUNOGEN
A recombinant human partial protein corresponding to amino acids 167-261 was used as the immunogen for the Prohibitin antibody.
STORAGE BUFFER
1 mg/ml in 1X PBS; BSA free, sodium azide free
APPLICATION DETAILS
Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
APPLICATION NOTES
Optimal dilution of the antibody should be determined by the researcher.
LOCALIZATION
Cytoplasmic
Questions & Answers (0)
Citations (0)
Immunofluorescence staining of human HepG2 cells with Prohibitin antibody (green, clone PHB/3225) and Reddot nuclear stain (red).
Immunofluorescence staining of human HepG2 cells with Prohibitin antibody (green, clone PHB/3225) and Reddot nuclear stain (red).
IHC testing of FFPE human liver tissue with Prohibitin antibody (clone PHB/3225). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
+3
IHC testing of FFPE human liver tissue with Prohibitin antibody (clone PHB/3225). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the Prohibitin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This Prohibitin antibody is available for research use only.
Regulatory & Compliance
Badge
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