POLR2A Antibody / RNA polymerase II subunit RPB1 / Phosphorylated Serine 5
SKU / CAT#: V7525-20UG
$21900
usd
$10.95/UG
V7525-20UG

POLR2A Antibody / RNA polymerase II subunit RPB1 / Phosphorylated Serine 5

NSJ Bioreagents
SKU / CAT#: V7525-20UG
MPN: V7525-20UG
$21900
usd
$10.95/UG
Available
SHIPS Jul 14th
Available
SHIPS Jul 14th
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Description
RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic...
RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the phospho-RNA polymerase II antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This phospho-RNA polymerase II antibody is available for research use only.
Regulatory & Compliance
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Specifications
CLASS
Primary Antibodies
SUBCLASS
Monoclonal
UNIPROT ID
P24928
CLONE NUMBER
CTD 8A7
*USAGE / SAFETY STATEMENT
This phospho-RNA polymerase II antibody is available for research use only.
FORMAT
Purified
APPLICATION.1
FACS, IF, WB, IHC-P
HOST SPECIES
Mouse
ISOTYPE
Mouse IgM, kappa
REACTIVITY SPECIES
Human
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
PEG precipitation
IMMUNOGEN
The immunogen for this phospho-RNA polymerase II antibody was 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-S5.
STORAGE BUFFER
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
APPLICATION DETAILS
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
APPLICATION NOTES
Optimal dilution of the recombinant phospho-RNA polymerase II antibody should be determined by the researcher.<br><br>1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
LOCALIZATION
Nuclear
Questions & Answers (0)
Citations (0)
IHC staining of FFPE human colon carcinoma with phospho-RNA polymerase II antibody (clone CTD 8A7). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human colon carcinoma with phospho-RNA polymerase II antibody (clone CTD 8A7). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Western blot testing of human HepG2, K562, and SK-BR-3 cell lysate with phospho-RNA polymerase II antibody (clone CTD 8A7). Routinely observed molecular weight: 200-250 kDa.
IHC staining of FFPE human colon carcinoma with phospho-RNA polymerase II antibody (clone CTD 8A7). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the phospho-RNA polymerase II antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This phospho-RNA polymerase II antibody is available for research use only.
Regulatory & Compliance
Badge
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