SM22/Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart.
SM22/Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart.
Shipping & Handling
Shipped In
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Safety & Storage
Storage Temperature
Store the SM22 alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This SM22 alpha antibody is available for research use only.
This SM22 alpha antibody is available for research use only.
FORMAT
Purified
APPLICATION.1
IHC-P
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG1, kappa
REACTIVITY SPECIES
Human
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
Protein G affinity chromatography
IMMUNOGEN
Recombinant full-length human protein was used as the immunogen for the SM22 alpha antibody. (3)
STORAGE BUFFER
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
APPLICATION DETAILS
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)
APPLICATION NOTES
Titering of the SM22 alpha antibody may be required for optimal performance.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.<br>3. Cross-reaction with SM22 beta protein has not been determined.
LOCALIZATION
Cytoplasmic
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Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the SM22 alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This SM22 alpha antibody is available for research use only.
Regulatory & Compliance
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