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POU2AF1 Antibody / BOB-1
SKU / CAT#: V3902-20UG
$21900
usd
$10.95/UG
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  4. POU2AF1 Antibody / BOB-1
V3902-20UG

POU2AF1 Antibody / BOB-1

NSJ Bioreagents
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SKU / CAT#: V3902-20UG
MPN: V3902-20UG
$21900
usd
$10.95/UG
Available
SHIPS May 16th
Available
SHIPS May 16th
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Description
BOB-1, also called POU2AF1, expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB-1. Conversely, only some cases of classical Hodgkin lymphoma show BOB-1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells. Expression of BOB-1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of...
BOB-1, also called POU2AF1, expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB-1. Conversely, only some cases of classical Hodgkin lymphoma show BOB-1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells. Expression of BOB-1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of...
Shipping & Handling
Shipped In
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Safety & Storage
Storage Temperature
Store the BOB-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This BOB-1 antibody is available for research use only.
Regulatory & Compliance
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Technical Documents
Datasheet (1)
nLyJcbob-1-antibody-bob1-2424-protein-microarray-specificity-validated-v3902
Specifications
CLASS
Primary Antibodies
SUBCLASS
Monoclonal
UNIPROT ID
Q16633
CLONE NUMBER
BOB1/2424
*USAGE / SAFETY STATEMENT
This BOB-1 antibody is available for research use only.
FORMAT
Purified
APPLICATION.1
FACS, IF, WB, IHC-P
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG2b, kappa
REACTIVITY SPECIES
Human
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
Protein G affinity
IMMUNOGEN
A portion of amino acids 148-255 from the human protein was used as the immunogen for the BOB-1 antibody.
STORAGE BUFFER
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
APPLICATION DETAILS
Flow cytometry: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
APPLICATION NOTES
Optimal dilution of the BOB-1 antibody should be determined by the researcher.
LOCALIZATION
Nuclear
Questions & Answers (0)
Citations (0)
IHC testing of FFPE human spleen with BOB-1 antibody (clone BOB1/2424). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human spleen with BOB-1 antibody (clone BOB1/2424). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human Raji cell lysate with BOB-1 antibody (clone BOB1/2424). Predicted molecular weight: ~28 kDa (unmodified), 35-40 kDa (ubiquitinated).
+3
Immunofluorescent staining of PFA-fixed human Raji cells with BOB-1 antibody (green, clone BOB1/2424) and Reddot nuclear stain.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the BOB-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This BOB-1 antibody is available for research use only.
Regulatory & Compliance
Badge
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