Calnexin Antibody
SKU / CAT#: V3795-100UG
$49900
usd
$4.99/UG
V3795-100UG

Calnexin Antibody

NSJ Bioreagents
SKU / CAT#: V3795-100UG
MPN: V3795-100UG
$49900
usd
$4.99/UG
Available
SHIPS Jul 24th
Available
SHIPS Jul 24th
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Description
It recognizes a protein of 90kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central...
It recognizes a protein of 90kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central...
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Shipped In
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Safety & Storage
Storage Temperature
Store the Calnexin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This Calnexin antibody is available for research use only.
Regulatory & Compliance
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Technical Documents
Specifications
CLASS
Primary Antibodies
SUBCLASS
Monoclonal
UNIPROT ID
P27824
CLONE NUMBER
CANX/1543
*USAGE / SAFETY STATEMENT
This Calnexin antibody is available for research use only.
FORMAT
Purified
APPLICATION.1
ELISA, WB, IHC-P
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG1, kappa
REACTIVITY SPECIES
Human
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
Protein G affinity chromatography
IMMUNOGEN
A portion of amino acids 1-300 from the human protein was used as the immunogen for the Calnexin antibody.
STORAGE BUFFER
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
APPLICATION DETAILS
ELISA: 2-4ug/ml (order BSA/azide-free format),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
APPLICATION NOTES
Titering of the Calnexin antibody may be required for optimal performance.
LOCALIZATION
Cytoplasmic
Questions & Answers (0)
Citations (0)
IHC testing of FFPE human renal cell carcinoma with Calnexin antibody (clone CANX/1543). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human renal cell carcinoma with Calnexin antibody (clone CANX/1543). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human 1) PANC-1 and 2) MCF7 cell lysate with Calnexin antibody (clone CANX/1543). Predicted molecular weight ~68 kDa but routinely observed at ~90 kDa.
+2
Western blot testing of human kidney lysate with Calnexin antibody (clone CANX/1543). Predicted molecular weight ~68 kDa but routinely observed at ~90 kDa.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the Calnexin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This Calnexin antibody is available for research use only.
Regulatory & Compliance
Badge
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