This mAb recognizes an ~170kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.

Shipping & Handling

Shipped In

Cold Packs

Safety & Storage

Storage Temperature

Store the TOP2A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Safety Statement

This TOP2A antibody is available for research use only.

Regulatory & Compliance

Technical Documents

Datasheet (1)

HFwVftop2a-antibody-top2a-1362-v3442

Specifications

CLASS

Primary Antibodies

SUBCLASS

Monoclonal

GENE ID

https://www.ncbi.nlm.nih.gov/gene/7153

UNIPROT ID

P11388

CLONE NUMBER

TOP2A/1362

*USAGE / SAFETY STATEMENT

This TOP2A antibody is available for research use only.

FORMAT

Purified

APPLICATION.1

WB, IF, IHC-P

HOST SPECIES

Mouse

ISOTYPE

Mouse IgG2b, kappa

REACTIVITY SPECIES

Human

CLONALITY

Monoclonal (mouse origin)

PURIFICATION

Protein G affinity chromatography

IMMUNOGEN

Amino acids 1352-1493 from the human protein were used as the immunogen for this TOP2A antibody.

STORAGE BUFFER

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

APPLICATION DETAILS

Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

APPLICATION NOTES

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TOP2A antibody to be titered up or down for optimal performance.

LOCALIZATION

Nuclear