Keratin-10 is a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. [Wiki]

Shipping & Handling

Shipped In

Cold Packs

Safety & Storage

Storage Temperature

Store the Cytokeratin 10 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Safety Statement

This Cytokeratin 10 antibody is available for research use only.

Regulatory & Compliance

Technical Documents

Datasheet (1)

nQsEMcytokeratin-10-antibody-de-k10-v3188

Specifications

CLASS

Primary Antibodies

SUBCLASS

Monoclonal

GENE ID

https://www.ncbi.nlm.nih.gov/gene/3858

CLONE NUMBER

DE-K10

*USAGE / SAFETY STATEMENT

This Cytokeratin 10 antibody is available for research use only.

FORMAT

Purified

APPLICATION.1

IHC-P

HOST SPECIES

Mouse

ISOTYPE

Mouse IgG1, kappa

REACTIVITY SPECIES

Human

CLONALITY

Monoclonal (mouse origin)

PURIFICATION

Protein G affinity chromatography

IMMUNOGEN

A cytoskeletal preparation from human ectocervical epithelium was used as the immunogen for this Cytokeratin 10 antibody.

STORAGE BUFFER

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

APPLICATION DETAILS

Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

APPLICATION NOTES

Optimal dilution of the anti-Keratin 10 antibody should be determined by the researcher.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

LOCALIZATION

Cytoplasmic