Myogenin Antibody / MYOG
SKU / CAT#: V3153-20UG
$21900
usd
$10.95/UG
V3153-20UG

Myogenin Antibody / MYOG

NSJ Bioreagents
SKU / CAT#: V3153-20UG
MPN: V3153-20UG
$21900
usd
$10.95/UG
Available
SHIPS Mar 19th
Available
SHIPS Mar 19th
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Description
Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei...
Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the Myogenin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This Myogenin antibody is available for research use only.
Regulatory & Compliance
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Technical Documents
Specifications
CLASS
Primary Antibodies
*USAGE / SAFETY STATEMENT
This Myogenin antibody is available for research use only.
FORMAT
Purified
SUBCLASS
Monoclonal
UNIPROT ID
P15173
CLONE NUMBER
F5D
APPLICATION.1
ELISA, IHC-P
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG1, kappa
REACTIVITY SPECIES
Human, Mouse, Rat
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
Protein G affinity chromatography
IMMUNOGEN
Amino acids 138-158 from the rat protein were used as the immunogen for this Myogenin antibody.
STORAGE BUFFER
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
APPLICATION DETAILS
ELISA (order BSA/sodium azide-free format for coating),Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
APPLICATION NOTES
The optimal dilution of the Myogenin antibody for each application should be determined by the researcher.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
LOCALIZATION
Nuclear
GENE ID
https://www.ncbi.nlm.nih.gov/gene/4656
Questions & Answers (0)
Citations (0)
IHC: Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with Myogenin antibody (F5D)
IHC: Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with Myogenin antibody (F5D)
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the Myogenin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This Myogenin antibody is available for research use only.
Regulatory & Compliance
Badge
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