It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Shipping & Handling

Shipped In

Cold Packs

Safety & Storage

Storage Temperature

Store the BrdU antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Safety Statement

This BrdU antibody is available for research use only.

Regulatory & Compliance

Technical Documents

Datasheet (1)

NDesNbrdu-antibody-brd-3-v3110

Specifications

CLASS

Primary Antibodies

*USAGE / SAFETY STATEMENT

This BrdU antibody is available for research use only.

FORMAT

Purified (Sodium Azide Free)

SUBCLASS

Monoclonal

CLONE NUMBER

BRD.3

APPLICATION.1

FACS, IF, IHC-P

HOST SPECIES

Mouse

ISOTYPE

Mouse IgG1, kappa

REACTIVITY SPECIES

All species

CLONALITY

Monoclonal (mouse origin)

PURIFICATION

Protein G affinity chromatography

IMMUNOGEN

Bromodeoxyuridine conjugated to BSA was used as the immunogen for the BrdU antibody.

STORAGE BUFFER

1 mg/ml in 1X PBS; BSA free, sodium azide free

APPLICATION DETAILS

Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT

APPLICATION NOTES

Optimal dilution of the BrdU antibody should be determined by the researcher.<p>1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 min at RT followed by digestion with Trypsin at 1mg/ml PBS, 10 min at 37oC.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

LOCALIZATION

Nuclear