This mAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This mAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed...
This mAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This mAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the Nuclear Marker antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This Nuclear Marker antibody is available for research use only.
This Nuclear Marker antibody is available for research use only.
FORMAT
Purified (Sodium Azide Free)
APPLICATION.1
IF, IHC-P
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG1, kappa
REACTIVITY SPECIES
Human, Rat
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
Protein G affinity chromatography
IMMUNOGEN
Nuclei of HL60 cells were used as the immunogen for the Nuclear Marker antibody.
STORAGE BUFFER
1 mg/ml in 1X PBS; BSA free, sodium azide free
APPLICATION DETAILS
Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
APPLICATION NOTES
Optimal dilution of the Nuclear Marker antibody should be determined by the researcher.<p>1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
LOCALIZATION
Nuclei
Questions & Answers (0)
Citations (0)
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Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the Nuclear Marker antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This Nuclear Marker antibody is available for research use only.
Regulatory & Compliance
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.