Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This mAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18...
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This mAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the Cytokeratin 8/18 antibody c°Cktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This Cytokeratin 8/18 antibody cocktail is available for research use only.
This Cytokeratin 8/18 antibody cocktail is available for research use only.
FORMAT
Purified
APPLICATION.1
IHC-P
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG1, kappa
REACTIVITY SPECIES
Human
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
Protein G affinity chromatography
IMMUNOGEN
Recombinant full-length human KRT8 protein (KRT8/803) and KRT18 protein (KRT18/835) were used as the immunogen for the Cytokeratin 8/18 antibody cocktail.
STORAGE BUFFER
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
APPLICATION DETAILS
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
APPLICATION NOTES
Optimal dilution of the Cytokeratin 8/18 antibody cocktail should be determined by the researcher.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
LOCALIZATION
Cytoplasmic
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Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the Cytokeratin 8/18 antibody c°Cktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This Cytokeratin 8/18 antibody cocktail is available for research use only.
Regulatory & Compliance
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