This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this mAb is completely blocked by the peptide used for raising this antibody. TGFa is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this mAb is completely blocked by the peptide used for raising this antibody. TGFa is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the TGF-alpha antibody c°Cktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This TGF-alpha antibody cocktail is available for research use only.
This TGF-alpha antibody cocktail is available for research use only.
FORMAT
Purified
APPLICATION.1
FACS, IF, IHC-P
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG1, kappa
REACTIVITY SPECIES
Human
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
PEG precipitation
IMMUNOGEN
Amino acids 34-43 (PPVAAAVVSH) from the human protein were used as the immunogen for the TGF-alpha antibody.
STORAGE BUFFER
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
APPLICATION DETAILS
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
APPLICATION NOTES
Optimal dilution of the TGF-alpha antibody should be determined by the researcher.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
LOCALIZATION
Cytoplasmic and extracellular (secreted)
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Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the TGF-alpha antibody c°Cktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This TGF-alpha antibody cocktail is available for research use only.
Regulatory & Compliance
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.