Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG), Lactadherin, or MFG-E8. HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.
Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG), Lactadherin, or MFG-E8. HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the MFGE8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This MFGE8 antibody is available for research use only.
This MFGE8 antibody is available for research use only.
FORMAT
Purified
APPLICATION.1
IHC-P
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG1, kappa
REACTIVITY SPECIES
Human
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
Protein G affinity chromatography
IMMUNOGEN
A human milk fat globule membrane preparation was used as the immunogen for the MFGE8 antibody.
STORAGE BUFFER
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
APPLICATION DETAILS
Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
APPLICATION NOTES
Optimal dilution of the MAP3K1 antibody should be determined by the researcher.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
LOCALIZATION
Cell surface & cytoplasmic
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Citations (0)
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Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the MFGE8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This MFGE8 antibody is available for research use only.
Regulatory & Compliance
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We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.