This mAb reacts with cells bearing HLA-A25 or HLA-Aw32 antigens. In addition, a reaction was observed with a cell of phenotype A2, Aw31; B17, Bw49. HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. HLA system comprises closely linked genes controlling highly polymorphic proteins involved in the presentation of peptides to the T-cell receptor, inhibition of NK cell cytotoxicity, and rejection of tissue allotransplantation. Specific alleles at HLA loci are associated with diseases. This mAb is specifically...
This mAb reacts with cells bearing HLA-A25 or HLA-Aw32 antigens. In addition, a reaction was observed with a cell of phenotype A2, Aw31; B17, Bw49. HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. HLA system comprises closely linked genes controlling highly polymorphic proteins involved in the presentation of peptides to the T-cell receptor, inhibition of NK cell cytotoxicity, and rejection of tissue allotransplantation. Specific alleles at HLA loci are associated with diseases. This mAb is specifically...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the HLA-A25 antibody (HLA-Aw32) at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This HLA-A25 antibody (HLA-Aw32) is available for research use only.
This HLA-A25 antibody (HLA-Aw32) is available for research use only.
FORMAT
Purified
APPLICATION.1
IHC-P
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG2a, kappa
REACTIVITY SPECIES
Human
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
Protein G affinity chromatography
IMMUNOGEN
Normal human peripheral blood lymphocytes of phenotype A1, Aw32, B7, B37, Cw-, Cw-, DR2, and DRw10 were used as the immunogen for the HLA-A25 antibody (HLA-Aw32).
STORAGE BUFFER
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
APPLICATION DETAILS
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
APPLICATION NOTES
Optimal dilution of the HLA-A25 antibody (HLA-Aw32) should be determined by the researcher.<p>1. Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
LOCALIZATION
Cell surface
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Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the HLA-A25 antibody (HLA-Aw32) at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This HLA-A25 antibody (HLA-Aw32) is available for research use only.
Regulatory & Compliance
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