Specifications
GENE ID
https://www.ncbi.nlm.nih.gov/gene/Not Applicable
*USAGE / SAFETY STATEMENT
This Phosphotyrosine antibody is available for research use only.
FORMAT
Purified (Sodium Azide Free)
APPLICATION.1
FACS, IF, WB, IP, IHC-P
ISOTYPE
Mouse IgG2b, kappa
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
Protein G affinity chromatography
IMMUNOGEN
Phosphotyrosine conjugated to KLH was used as the immunogen for this antibody.
STORAGE BUFFER
1 mg/ml in 1X PBS; BSA free, sodium azide free
APPLICATION DETAILS
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml,Immunoprecipitation: 1-2ug/500ug protein lysate,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
APPLICATION NOTES
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Phosphotyrosine antibody to be titered up or down for optimal performance.<p>1. No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.<br>
LOCALIZATION
Depends on phosphorylated target localization