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Phosphotyrosine Antibody / P-Tyr
SKU / CAT#: V2359-20UG
$21900
usd
$10.95/UG
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V2359-20UG

Phosphotyrosine Antibody / P-Tyr

NSJ Bioreagents
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SKU / CAT#: V2359-20UG
MPN: V2359-20UG
$21900
usd
$10.95/UG
Available
SHIPS May 29th
Available
SHIPS May 29th
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Description
Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing...
Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing...
Shipping & Handling
Shipped In
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Safety & Storage
Storage Temperature
Store the Phosphotyrosine antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This Phosphotyrosine antibody is available for research use only.
Regulatory & Compliance
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Technical Documents
Datasheet (1)
RGDqjphosphotyrosine-antibody-py20-v2359
Specifications
CLASS
Primary Antibodies
SUBCLASS
Monoclonal
GENE ID
https://www.ncbi.nlm.nih.gov/gene/Not Applicable
CLONE NUMBER
PY20
*USAGE / SAFETY STATEMENT
This Phosphotyrosine antibody is available for research use only.
FORMAT
Purified
APPLICATION.1
FACS, IF, WB, IP, IHC-P
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG2b, kappa
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
Protein G affinity chromatography
IMMUNOGEN
Phosphotyrosine conjugated to KLH was used as the immunogen for this antibody.
STORAGE BUFFER
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
APPLICATION DETAILS
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml,Immunoprecipitation: 1-2ug/500ug protein lysate,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
APPLICATION NOTES
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Phosphotyrosine antibody to be titered up or down for optimal performance.<p>1. No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.<br>
LOCALIZATION
Depends on phosphorylated target localization
Questions & Answers (0)
Citations (0)
IHC staining of breast carcinoma with phosphotyrosine antibody (PY20). Note cell surface and cytoplasmic staining.
IHC staining of breast carcinoma with phosphotyrosine antibody (PY20). Note cell surface and cytoplasmic staining.
SDS-PAGE Analysis of Purified, BSA-Free Phosphotyrosine Antibody (clone PY20). Confirmation of Integrity and Purity of the Antibody.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the Phosphotyrosine antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This Phosphotyrosine antibody is available for research use only.
Regulatory & Compliance
Badge
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