p40 (p63 delta) is a marker recently determined to be highly specific for squamous basal cells in the immunohistochemistry (IHC) application. The current more routinely recommended marker, p63, appears to have less specificity compared to p40, especially on squamous cell tumors. The ability to differentiate between lung adenocarcinoma vs. squamous cell carcinoma is difficult and has bearing on the different therapeutic avenues for each subtype treatment. p40 antibody�s ability to distinguish between the tumor types appears to be superior when compared to p63. The ability to utilize a p40 antibody...
p40 (p63 delta) is a marker recently determined to be highly specific for squamous basal cells in the immunohistochemistry (IHC) application. The current more routinely recommended marker, p63, appears to have less specificity compared to p40, especially on squamous cell tumors. The ability to differentiate between lung adenocarcinoma vs. squamous cell carcinoma is difficult and has bearing on the different therapeutic avenues for each subtype treatment. p40 antibody�s ability to distinguish between the tumor types appears to be superior when compared to p63. The ability to utilize a p40 antibody...
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Safety & Storage
Storage Temperature
Store the p40 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This p40 antibody is available for research use only.
This p40 antibody is available for research use only.
FORMAT
Purified
APPLICATION.1
WB, IHC-P
HOST SPECIES
Rabbit
ISOTYPE
Rabbit IgG
REACTIVITY SPECIES
Human, Mouse, Rat
CLONALITY
Polyclonal (rabbit origin)
PURIFICATION
Protein A affinity chromatography
IMMUNOGEN
Amino acids ENNAQTQFSEPQY (aa 5-17) of human p40 / p63 delta were used as the immunogen for this antibody.
STORAGE BUFFER
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
APPLICATION DETAILS
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
APPLICATION NOTES
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the p40 antibody to be titered up or down for optimal performance.<p>1. Staining of FFPE tissues requires boiling sections in 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
LOCALIZATION
Nuclear
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Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the p40 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This p40 antibody is available for research use only.
Regulatory & Compliance
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