Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Myogenin antibody labels the...
Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Myogenin antibody labels the...
Shipping & Handling
Shipped In
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Safety & Storage
Storage Temperature
Store the Myogenin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This Myogenin antibody is available for research use only.
This Myogenin antibody is available for research use only.
FORMAT
Purified
APPLICATION.1
IHC-P
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG1, kappa
REACTIVITY SPECIES
Human, Mouse, Rat
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
Protein G affinity chromatography
IMMUNOGEN
Human myogenin recombinant protein was used as the immunogen for this antibody.
STORAGE BUFFER
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
APPLICATION DETAILS
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
APPLICATION NOTES
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Myogenin antibody to be titered up or down for optimal performance.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
LOCALIZATION
Nuclear
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Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the Myogenin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This Myogenin antibody is available for research use only.
Regulatory & Compliance
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