This antibody recognizes a protein of 185kDa, which is identified as HER2 / ErbB2. Its epitope is localized in the extracellular domain. ErbB2 is a member of the EGFR family. This antibody is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. ErbB2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
This antibody recognizes a protein of 185kDa, which is identified as HER2 / ErbB2. Its epitope is localized in the extracellular domain. ErbB2 is a member of the EGFR family. This antibody is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. ErbB2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
Shipping & Handling
Shipped In
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Safety & Storage
Storage Temperature
Store the ErbB2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This ErbB2 antibody is available for research use only.
This ErbB2 antibody is available for research use only.
FORMAT
Purified
SUBCLASS
Monoclonal
CLONE NUMBER
HRB2/258
APPLICATION.1
ELISA, FACS, IF
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG2a, kappa
REACTIVITY SPECIES
Human, Rat
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
Protein G purified
IMMUNOGEN
Recombinant human protein was used as the immunogen for this ErbB2 antibody.(1)
STORAGE BUFFER
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
APPLICATION DETAILS
ELISA (order BSA/sodium azide-free format for coating),Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml
APPLICATION NOTES
Variations in protocols, secondaries and substrates may require the antibody to be titered for optimal performance.<p>1. This ErbB2 antibody binds to the extracellular/cell surface region of the protein.
LOCALIZATION
Extracellular/Intracellular cell membrane
GENE ID
https://www.ncbi.nlm.nih.gov/gene/2064 (Human)
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Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the ErbB2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This ErbB2 antibody is available for research use only.
Regulatory & Compliance
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We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.