Regulator of nonsense transcripts 2 is a protein that in humans is encoded by the UPF2 gene. This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins...

Shipping & Handling

Shipped In

Cold Packs

Safety & Storage

Storage Temperature

After reconstitution, the hUPF2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Safety Statement

This hUPF2 antibody is available for research use only.

Regulatory & Compliance

Technical Documents

Datasheet (1)

WQjRdhupf2-antibody-rent2-rq6694

Specifications

CLASS

Primary Antibodies

SUBCLASS

Polyclonal

UNIPROT ID

Q9HAU5

*USAGE / SAFETY STATEMENT

This hUPF2 antibody is available for research use only.

FORMAT

Antigen affinity purified

APPLICATION.1

WB, IHC-P, FACS, IF, Direct ELISA

HOST SPECIES

Rabbit

ISOTYPE

Rabbit IgG

REACTIVITY SPECIES

Human

CLONALITY

Polyclonal (rabbit origin)

PURIFICATION

Antigen affinity purified

IMMUNOGEN

Recombinant human protein (amino acids K200-H651) was used as the immunogen for the hUPF2 antibody.

STORAGE BUFFER

0.5mg/ml if reconstituted with 0.2ml sterile DI water

APPLICATION DETAILS

Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml

APPLICATION NOTES

Optimal dilution of the hUPF2 antibody should be determined by the researcher.

LOCALIZATION

Cytoplasmic

BUFFER

Lyophilized from 1X PBS with 2% Trehalose

Questions & Answers (0)

Citations (0)

Immunofluorescent staining of FFPE human A431 cells with hUPF2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

IHC staining of FFPE human appendiceal adenocarcinoma tissue with hUPF2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

+2

IHC staining of FFPE human liver cancer tissue with hUPF2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Shipping & Handling

Shipped In

Cold Packs

Safety & Storage

Storage Temperature

After reconstitution, the hUPF2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Safety Statement

This hUPF2 antibody is available for research use only.

Regulatory & Compliance

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