PLK1 Antibody
SKU / CAT#: R32133
$41900
usd
$4.19/UG
R32133

PLK1 Antibody

NSJ Bioreagents
$41900
usd
$4.19/UG
Available
SHIPS Mar 17th
Available
SHIPS Mar 17th
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Description
Serine/threonine-protein kinase PLK1, also known as polo-like kinase 1 (PLK-1) or serine/threonine-protein kinase 13 (STPK13), is an enzyme that in humans is encoded by the PLK1 (polo-like kinase 1) gene. Plk1 is an early trigger for G2/M transition. It supports the functional maturation of the centrosome in late G2/early prophaseand establishment of the bipolar spindle. Also, Plk1 phosphorylates and activates cdc25C, a phosphatase that dephosphorylates and activates the cyclinB/cdc2 complex. Studies have shown that the loss of PLK1 expression can inducepro-apoptotic pathways and inhibit growth....
Serine/threonine-protein kinase PLK1, also known as polo-like kinase 1 (PLK-1) or serine/threonine-protein kinase 13 (STPK13), is an enzyme that in humans is encoded by the PLK1 (polo-like kinase 1) gene. Plk1 is an early trigger for G2/M transition. It supports the functional maturation of the centrosome in late G2/early prophaseand establishment of the bipolar spindle. Also, Plk1 phosphorylates and activates cdc25C, a phosphatase that dephosphorylates and activates the cyclinB/cdc2 complex. Studies have shown that the loss of PLK1 expression can inducepro-apoptotic pathways and inhibit growth....
Shipping & Handling
Shipped In
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Safety & Storage
Storage Temperature
After reconstitution, the PLK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Safety Statement
This PLK1 antibody is available for research use only.
Regulatory & Compliance
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Technical Documents
Specifications
CLASS
Primary Antibodies
SUBCLASS
Polyclonal
UNIPROT ID
P53350
*USAGE / SAFETY STATEMENT
This PLK1 antibody is available for research use only.
FORMAT
Antigen affinity purified
APPLICATION.1
WB, IHC-P
HOST SPECIES
Rabbit
ISOTYPE
Rabbit IgG
REACTIVITY SPECIES
Human, Mouse, Rat
CLONALITY
Polyclonal (rabbit origin)
PURIFICATION
Antigen affinity
IMMUNOGEN
Amino acids 86-430 of human PLK1 were used as the immunogen for the PLK1 antibody.
STORAGE BUFFER
0.5mg/ml if reconstituted with 0.2ml sterile DI water
APPLICATION DETAILS
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
APPLICATION NOTES
Optimal dilution of the PLK1 antibody should be determined by the researcher.
LOCALIZATION
Nuclear, minor cytoplasmic
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Citations (0)
Western blot testing of human 1) HeLa and 2) PANC cell lysate with PLK1 antibody. Predicted/observed molecular weight ~68 kDa.
Western blot testing of human 1) HeLa and 2) PANC cell lysate with PLK1 antibody. Predicted/observed molecular weight ~68 kDa.
IHC testing of FFPE human lung cancer with PLK1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
+1
IHC testing of FFPE mouse lymph node with PLK1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
After reconstitution, the PLK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Safety Statement
This PLK1 antibody is available for research use only.
Regulatory & Compliance
Badge
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