Interferon-induced, double-stranded RNA-activated protein kinase is a serine-threonine kinase. Activation by dsRNAs leads to autophosphorylation of PRKR and allows the kinase to phosphorylate its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor-2 (EIF2-alpha), leading to the inhibition of protein synthesis. Human gamma-interferon (IFNG) mRNA exploits localized activation of PRKR in the cell to regulate its own translation. IFNG mRNA activates PRKR through a pseudoknot in its 5-prime untranslated region. The HCV envelope protein E2 contains a sequence identical...
Interferon-induced, double-stranded RNA-activated protein kinase is a serine-threonine kinase. Activation by dsRNAs leads to autophosphorylation of PRKR and allows the kinase to phosphorylate its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor-2 (EIF2-alpha), leading to the inhibition of protein synthesis. Human gamma-interferon (IFNG) mRNA exploits localized activation of PRKR in the cell to regulate its own translation. IFNG mRNA activates PRKR through a pseudoknot in its 5-prime untranslated region. The HCV envelope protein E2 contains a sequence identical...
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Shipped In
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Safety & Storage
Storage Temperature
Aliquot the PRKR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Safety Statement
This PRKR antibody is available for research use only.
This PRKR antibody is available for research use only.
FORMAT
Purified
APPLICATION.1
WB, IHC, ELISA
HOST SPECIES
Rabbit
ISOTYPE
Rabbit Ig
REACTIVITY SPECIES
Human, Mouse
CLONALITY
Polyclonal (rabbit origin)
PURIFICATION
Purified
IMMUNOGEN
A portion of amino acids 519-550 from the human protein was used as the immunogen for this PRKR antibody.
STORAGE BUFFER
In 1X PBS pH 7.4 with 0.09% sodium azide
APPLICATION DETAILS
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
APPLICATION NOTES
The stated application concentrations are suggested starting amounts. Titration of the PRKR antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Aliquot the PRKR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Safety Statement
This PRKR antibody is available for research use only.
Regulatory & Compliance
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We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.