The rabbit antibody for VIP is generated against porcine VIP conjugated to bovine thyroglobulin with carbodiimide. The antibody is provided as 100 µL of lyophilized whole serum, and 0.09% sodium azide.
The rabbit antibody for VIP is generated against porcine VIP conjugated to bovine thyroglobulin with carbodiimide. The antibody is provided as 100 µL of lyophilized whole serum, and 0.09% sodium azide.
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Shipped In
Ambient
Safety & Storage
Storage Temperature
-20°C
Safety Statement
This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records. This product is intended only for laboratory research and development purposes.
Regulatory & Compliance
Specifications
CLASS
Primary
COUNTRY OF ORIGIN
United States of America
*USAGE / SAFETY STATEMENT
This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records. This product is intended only for laboratory research and development purposes.
REGULATORY NOTICE & RESTRICTIONS
For Laboratory Reagent Use Only. Analytical and performance characteristics are not established. THIS PRODUCT IS FOR RESEARCH USE ONLY AND IS NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE.
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Reviews & Ratings (2)
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We have used this antibody to stain for VIP positive neurons (also stains processes) in frozen tissue sections and whole mount preparations of mouse intestine....
Meenakshi
Columbia University Medical Center
After failed several experiments by using other antibodies, we applied VIP antibody (20077) for mouse brain tissue sections for suprachiasmatic nucleus immunohistochemistry....
David
BioTeq Corporation
We have used this antibody to stain for VIP positive neurons (also stains processes) in frozen tissue sections and whole mount preparations of mouse intestine....
Meenakshi
Columbia University Medical Center
After failed several experiments by using other antibodies, we applied VIP antibody (20077) for mouse brain tissue sections for suprachiasmatic nucleus immunohistochemistry....
David
BioTeq Corporation
Works Great!
Meenakshi R.
Columbia University Medical Center
We have used this antibody to stain for VIP positive neurons (also stains processes) in frozen tissue sections and whole mount preparations of mouse intestine. We get consistent staining at 1:750. Tissue prep: 4% PFA/PBS for 1.5 hrs, cryoprotected in 30% sucrose until they sink, OCT embedded, and sectioned. For whole mounts, we open up intestine and fix in 4% PFA/PBS for 1.5 hrs.
IF protocol:
Sections: Rehydrate in PBS, block with HINGS, stain in primary @4C O/N.
Wmts: Wash in PBST, stain in primary with HINGS and DMSO @4C 48 hrs.
Sections: Wash with PBS, stain with Alexafluor secondaries (Invitrogen), wash with PBS, and DAPI mount.
Wmts: Wash in PBST, stain in secondary with HINGS and DMSO @RT 48 hrs.
Optional: proceed with MeOH dehydration and optical clearing.
Worked like a charm
David M.
BioTeq Corporation
After failed several experiments by using other antibodies, we applied VIP antibody (20077) for mouse brain tissue sections for suprachiasmatic nucleus immunohistochemistry. It turned out that this antibody worked like a charm on my frozen sections. This is definitely an excellent VIP antibody.
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Shipping & Handling
Shipped In
Ambient
Safety & Storage
Storage Temperature
-20°C
Safety Statement
This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records. This product is intended only for laboratory research and development purposes.
Regulatory & Compliance
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