The HighPrep™ PCR Clean-up System is a paramagnetic beads-based post PCR clean-up reagent designed for an efficient purification of DNA fragments and tight size-specific selection of amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, adapoter ligation,...
The HighPrep™ PCR Clean-up System is a paramagnetic beads-based post PCR clean-up reagent designed for an efficient purification of DNA fragments and tight size-specific selection of amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, adapoter ligation,...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store at 4°C to 8°C | DO NOT FREEZE
Safety Statement
For Research Use Only. Not for Use in Diagnostic Procedures.
Post-PCR and post-enzymatic reaction clean-up used for/during: NGS library preparation, Amplicon size selection for NGS, Microarrays, PCR, Restriction enzyme digestions, adapter ligations, Cloning
USAGE
This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS,
Add HighPrep™ PCR reagent to the reaction mixture,
Bind DNA to paramagnetic beads,
Separation of beads + DNR from contaminants,
Wash beads with Ethanol to remove contaminants,
Elute purified PCR products or other DNA fragments from beads
*USAGE / SAFETY STATEMENT
For Research Use Only. Not for Use in Diagnostic Procedures.
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